Biogas is an environmentally advantageous energy source which is mostly comprised of CH4 (60%) and CO2 (35–40%) in addition to NH3, H2S, H2, O2, N2, and CO. Biogas is the gas that is evolved from anaerobic digestion, for the transformation of waste materials to energy sources through the treatment of various organic waste such as municipal solid waste, food waste, industrial waste, sewage sludge, animal manure and agricultural residues, which is known as biomass. The anaerobic digestion of different feedstocks is one of the most promising ways to meet the European objectives. In fact, the conversion into methane depends on the amount of basic organic components (fats, proteins, carbohydrates), on the percentage of the dry substance and volatile solids present in the dry matter. In this research, several biomasses are characterized through the analysis of total solids, organic substance and elementary analysis (CHNSO analysis). Agro-industrial wastes, agricultural residues, livestock wastes are the analyzed biomasses and these results as m3-biogas/t-biomass are obtained from: Candies 231, carrots 27, corn 221, barley 219, tomatoes 9, watermelon 12, pork (50%)-candies (50%) 186, corn (50%)-candies (50%) 226, pork (50%)-tomatoes (50) 75, bovine 62, pork 142. Results show that, substrates such as candies have a high content of organic substance, so they have a high potential to produce biogas but having the high content of total solids it is necessary to be co-digested. Hence, it is concluded that biomasses with higher carbon content has the higher yield of methane which are basically from livestock wastes, while lower levels are obtained for agro-industrial wastes.rnrn